金钱草组方对犬草酸钙尿结石形成的影响

doi:10.11843/j.issn.0366-6964.2015.10.021

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (10): 1867-1874.doi: 10.11843/j.issn.0366-6964.2015.10.021

金钱草组方对犬草酸钙尿结石形成的影响

何钉玲，胡芳芳，苏永康，邱先帅，肖熹玉，潘家强^*

（华南农业大学兽医学院，广州 510642）

收稿日期:2015-01-21 出版日期:2015-10-23 发布日期:2015-10-23
通讯作者: 潘家强，E-mail：panjq@scau.edu.cn
作者简介:何钉玲（1989-），女，湖南邵阳人，硕士生，主要从事临床内科学方面的研究，E-mail：756424826@qq.com
基金资助:
广东省兽医临床重大疾病综合防控重点实验室开放课题（2013A061401013）

Effect of Jinqiancao Compound Potion on the Formation of Calcium Oxalate Stones in the Urine of Canine

HE Ding-ling，HU Fang-fang，SU Yong-kang，QIU Xian-shuai，XIAO Xi-yu，PAN Jia-qiang^*

(College of Veterinary Medicine，South China Agricultural University，Guangzhou 510642，China)

Received:2015-01-21 Online:2015-10-23 Published:2015-10-23

摘要/Abstract

摘要：

研究金钱草组方汤剂对试验性犬草酸钙尿结石形成的影响，初步探讨其作用机制。12只雄性中华田园犬随机分为3组，分别为对照组、模型组和治疗组。对照组饲喂基础日粮，模型组和治疗组在饲喂基础日粮的基础上，饮食中添加乙二醇、氯化铵、碳酸钙和维生素D。待试验犬尿液沉渣在显微镜检视野下出现80%的草酸钙结晶后，给治疗组犬灌服金钱草组方汤剂。试验过程中检测试验犬尿液中微量白蛋白（mAlb）含量、N-乙酰-β-D氨基葡萄糖苷酶（NAG酶）活力、γ-谷氨酰转移酶（γ-GT酶）活力；检测血清中肌酐（Crea）、尿素氮（BUN）、尿酸（UA）、丙二醛（MDA）含量和乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）活力。结果显示：与模型组相比，金钱草组方汤剂能显著降低治疗组犬尿液中NAG酶和γ-GT酶活力；降低治疗组犬血清中UA、MDA含量和LDH酶活力，提高SOD酶活力。金钱草组方汤剂能有效降低草酸钙结石形成过程对肾功能的损伤，提高血液的抗氧化酶活性，即能有效修复肾细胞损伤，保证肾的作用，防止犬草酸钙尿结石的形成。

Abstract:

The experiment was designed to preliminary study on the effect of Jinqiancao compound potion to the formation of calcium oxalate stones in the urine of canine.Twelve healthy male Canis Lupus familiaris were randomly divided into 3 groups，which were the control group，the model group and the treatment group.The dogs in control group were fed with basal diet.The dogs in model group and treatment group were fed with basal diet plus glycol，ammonium chloride，calcium carbonate and vitamin D.Once 80% of the microscope view appeared calcium oxalate crystals in the urine of the experimental dogs，the Jinqiancao compound potion was orally administrated to the dogs of treatment group.During the experiment，the urine index(including the content of microalbumin，the enzymatic activity of N-acetyl-β-D-Glucosaminidase and γ-glutamyltransferase) and the serum index(including the content of creatinine，blood urea nitrogen，uric acid and malondialdehyde，the enzymatic activity of lactate dehydrogenase and superoxide dismutase) were detected.Results were as follows：compared with model group，by treating Jinqiancao compound potion，the treatment group appeared lower activity of N-acetyl-β-D-Glucosaminidase，lower activity of γ-glutamyltransferase，lower concent of serum uric acid，lower malondialdehyde，lower activity of lactate dehydrogenase and higher activity of serum superoxide dismutase than that of modle group.Our study demonstrates that Jinqiancao compound can effectively reduce the damage to renal during the formation of calcium oxalate calculus，improve the enzyme activity of blood antioxidant.In other words，kidney cells were effectively repaired to ensure the function of kidney by the Jinqiancao compound，which to prevent the formation of calcium oxalate calculus in the urine of canine.

中图分类号:

S856.59

何钉玲，胡芳芳，苏永康，邱先帅，肖熹玉，潘家强. 金钱草组方对犬草酸钙尿结石形成的影响[J]. 畜牧兽医学报, 2015, 46(10): 1867-1874.

HE Ding-ling，HU Fang-fang，SU Yong-kang，QIU Xian-shuai，XIAO Xi-yu，PAN Jia-qiang. Effect of Jinqiancao Compound Potion on the Formation of Calcium Oxalate Stones in the Urine of Canine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1867-1874.

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金钱草组方对犬草酸钙尿结石形成的影响

Effect of Jinqiancao Compound Potion on the Formation of Calcium Oxalate Stones in the Urine of Canine

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